Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

P1415

Sigma-Aldrich

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

Nível de qualidade

200

forma

liquid (2x concentrate)

atividade externa

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temperatura de armazenamento

−20°C

Descrição geral

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Aplicação

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Componentes

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
  • 200 μg/mL DNA
  • 2mg Bovine Serum Albumin (BSA)
  • 2mg Ficoll
  • 2mg Polyvinylpyrrolidone (PVP)
  • 0.15 M sodium citrate
  • 1.5M NaCl

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 7

1 of 7

Sigma-Aldrich

Sigma-Aldrich

S2015

SSPE Buffer 20× Concentrate

SSC Buffer 20x for Northern and Southern blotting, solution

Sigma-Aldrich

93017

SSC Buffer 20x

SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

S6639

SSC Buffer 20× Concentrate

Sigma-Aldrich

Sigma-Aldrich

S0902

SSC Buffer

DIG Easy Hyb™ suitable for hybridization, solution

Roche

11603558001

DIG Easy Hyb™

Sigma-Aldrich

Sigma-Aldrich

SRE0068

SSC Buffer, 20X Concentrate

Reagente de bloqueio For nucleic acid hybridization and detection

Roche

11096176001

Reagente de bloqueio

T M Lau et al.
Human reproduction (Oxford, England), 11(12), 2629-2634 (1996-12-01)
The expression of endometrial progesterone receptor mRNA during the human menstrual cycle and in Norplant users was studied using digoxigenin-labelled ribonucleic probes for in-situ hybridization on 6 microns paraffin embedded endometrial sections. The staining intensity was scored blind semi-quantitatively. Blood
Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Andrew M Pilling et al.
Toxicologic pathology, 35(3), 348-355 (2007-04-25)
Spontaneous follicular and C-cell tumors of the thyroid gland in the Han Wistar rat were examined using two morphologic procedures. Firstly, in situ hybridization (ISH) was used to localize thyroglobulin (TG) and calcitonin (CT) mRNAs. Secondly, the proteins for these
Jianmin Su et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(39), 7421-7435 (2020-08-28)
GABAergic interneurons represent a heterogenous group of cell types in neocortex that can be clustered based on developmental origin, morphology, physiology, and connectivity. Two abundant populations of cortical GABAergic interneurons include the low-threshold, somatostatin (SST)-expressing cells and the fast-spiking, parvalbumin

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica