R6750
Ribonucleic acid from baker′s yeast (S. cerevisiae)
Sinônimo(s):
RNA
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Nível de qualidade
temperatura de armazenamento
−20°C
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Descrição geral
Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).
Aplicação
Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.
Nota de preparo
Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
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Protocolos
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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