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Isocitric Dehydrogenase (NADP) from porcine heart

Name: Isocitric Dehydrogenase (NADP) from porcine heart
Brand: SIGMA

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Synonym(s):

threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH

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About This Item

CAS Number:

9028-48-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

200-289-5

MDL number:

MFCD00131420

EC Number:

1.1.1.42 (BRENDA, IUBMB)

Specific activity:

3-20 units/mg protein

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Properties

type

Type IV

Quality Level

200

form

buffered aqueous glycerol solution

quality

≤ 0.2% of main activity is obtained with NAD as cofactor.

specific activity

3-20 units/mg protein

composition

Protein biuret

shipped in

wet ice

storage temp.

2-8°C

Description

Application

Isocitric dehydrogenase (IDH) has been used:

for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway[1]
as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
in isocitrate assay[2]

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.[3]

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

Other Notes

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

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This Item	I1877	M2634	SAB2501739
Sigma-Aldrich I2002 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich I1877 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart	Sigma-Aldrich SAB2501739 Anti-IDH3G antibody produced in goat
specific activity 3-20 units/mg protein	specific activity 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)	specific activity 600-1000 units/mg protein (biuret)	specific activity -
form buffered aqueous glycerol solution	form solid	form buffered aqueous glycerol solution	form buffered aqueous solution
shipped in wet ice	shipped in -	shipped in wet ice	shipped in wet ice
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
quality ≤ 0.2% of main activity is obtained with NAD as cofactor.	quality -	quality -	quality -

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Storage Class

10 - Combustible liquids

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WGK 2

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Interactions between transhydrogenase and thio-nicotinamide Analogues of NAD(H) and NADP(H) underline the importance of nucleotide conformational changes in coupling to proton translocation.

Avtar Singh et al.

The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)

Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding

Icksoo Lee et al.

Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)

Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that

Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5.

Erdem M Terzi et al.

Science advances, 7(22) (2021-05-28)

Intracellular iron levels are strictly regulated to support homeostasis and avoid iron-mediated ROS production. Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis.

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Global Trade Item Number

SKU	GTIN
I2002-25MG	04061832730448
I2002-250MG	04061832730431
I2002-25G-A	04061833848241
I2002-100MG	04061833848234
I2002-500MG	04061832730455
I2002-5G-A	04061832490205

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