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D3035

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

Deoxyribonucleic acid from human placenta

Synonym(s):

DNA

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About This Item

CAS Number:
9007-49-2
UNSPSC Code:
41106310
NACRES:
NA.52
MDL number:
MFCD00130921
eCl@ss:
32160414
Biological source:
human placenta
Form:
buffered aqueous solution
Storage temp.:
−20°C
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biological source

human placenta

Quality Level

200

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods[1]
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines[2]
  • as an internal control to estimate the degree of fragmentation of circulating DNA[3]
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11[4]
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species[5]
  • to calculate copy number of genes, relative to normal human tissue[6]
  • in PCR reactions[7]
For use in Southern hybridizations.

Other Notes

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

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This Item
262012D4154D3404
Deoxyribonucleic acid from human placenta buffered aqueous solution, sexed, female

Sigma-Aldrich

D3035

Deoxyribonucleic acid from human placenta

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes Purified DNA from salmon testes. Absorption (0.001% solution in phosphate buffer, pH 7.0, 260 nm): ≥0.155.

Millipore

262012

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes

Plasmid DNA from E. coli RRI pUC18, buffered aqueous solution

Sigma-Aldrich

D4154

Plasmid DNA from E. coli RRI

Plasmid DNA from E. coli RRI pUC19, buffered aqueous solution

Sigma-Aldrich

D3404

Plasmid DNA from E. coli RRI

grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

Molecular Biology

biological source

human placenta

biological source

-

biological source

Escherichia coli (RRI)

biological source

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

buffered aqueous solution

form

solid

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

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wgk

WGK 3

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
123K3797
123K37971
089K3786
089K3789
089K3788

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Related Content

Product Information Sheet - D3035

Product Information Sheet

Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Joanne S Aveyard et al.
The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)
Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
View All Related Papers

Global Trade Item Number

SKUGTIN
D3035-.1MG04061833562925
D3035-.5MG04061833420430
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Questions

  1. Hi, Could you please clarify what is the concentration (and the volume) of the D3035 product. Thank you

    1 answer

    1. The concentration will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate:
      https://www.sigmaaldrich.com/product/sigma/d3035#product-documentation

      The volume will depend on the pack size ordered and the concentration.
      For instance, a 0.5 mg pack at 0.05 mg/ml will have a volume of at least 10ml.

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