This product is quite stable when stored in tablet form at 2-8°C. While storing the tablets at 20°C is not expected to be detrimental, stability studies have not been conducted. Upon reconstitution, prepared solutions are stable at least 2 weeks at 2-8°C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/291/s8830dat-ms.pdf
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
2 tabs | Warenkorb auf Verfügbarkeit prüfen | € 73,30 |
20 tabs | Warenkorb auf Verfügbarkeit prüfen | € 664,00 |
Über diesen Artikel
Produktname
SIGMAFAST™ Proteasehemmer-Cocktail-Tabletten, EDTA-frei, for use in purification of Histidine-tagged proteins
biological source
bovine lung
Quality Segment
form
tablet
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Features and Benefits
Pepstatin A: Enthält eine einzigartige, proprietäre Formulierung, die für zusätzlichen Schutz, der in Tablettenform normalerweise nicht erhalten wird, ein Pepstatin-A-Salz enthält.
Breite Spezifizität: Hemmt Serin-, Cystein-, Aspartat- und Metallproteasen, wodurch ein breiter Schutz für Proteinproben bereitgestellt wird.
Hohe Ausbeute: Eine Tablette erzeugt 100 ml Cocktail und wird zur Hemmung der Proteasen in maximal 20 g Zellmasse in 100 ml Extrakt empfohlen.
Lange Stabilität: Die Tabletten sind, wie geliefert, mindestens 2 Jahre bei 2–8 °C haltbar. Die rekonstituierte Proteaseinhibitor-Lösung (1´ oder 10´) ist mindestens 2 Wochen bei 2–8 °C haltbar.
Physical form
Preparation Note
Other Notes
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
Legal Information
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| form tablet | form tablet | form DMSO solution | form tablet |
| biological source bovine lung | biological source bovine lung, bovine milk | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
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Danger
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Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Lagerklasse
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Can the tablets be stored at -20C? Any stability data around this?
1 answer-
Helpful?
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Can we use S8830-SIGMA FAST, EDTA-Free, tablet for plant extract also?
1 answer-
This product has not been tested for use with plants. For applications involving plants, product P9599, Protease Inhibitor Cocktail, is recommended for plant cell and tissue extracts.
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Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
1 answer-
The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
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Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
1 answer-
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
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How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
1 answer-
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
1 answer-
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
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What is the difference between Protease Inhibitors P8849 and S8830?
1 answer-
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
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