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R8758

Sigma-Aldrich

RPMI-1640-Medium

RPMI-1640 Medium

With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL-Nummer:
MFCD00217820
UNSPSC-Code:
12352207
NACRES:
NA.75
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

RPMI-1640-Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Qualitätsniveau

400

Sterilität

sterile-filtered

Form

liquid

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Komponenten

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

RPMI-1640 Medium wurde 1966 im Roswell Park Memorial Institute von Moore und seinen Kollegen entwickelt. Als Modifikation des McCoy-5A-Mediums wurde es für lymphoblastoide Zellen in Suspensionskultur formuliert, hat sich aber seitdem auch für viele adhärente Zellen als geeignet erwiesen.
RPMI 1640-Medium wurde 1966 am Roswell Park Memorial Institute von Moore und seinen Kollegen entwickelt. Als eine Modifikation von McCoy′s 5A-Medium wurde es formuliert, um lymphoblastoide Zellen in Suspensionskultur zu ernähren, aber seither wurde aufgezeigt, dass es ein breites Spektrum von Zellen ernährt, die verankerungsabhängig sind. Ursprünglich war es dazu gedacht, mit einer Serumsergänzung verwendet zu werden, doch es wurde aufgezeigt, dass RPMI 1640 verschiedene Zelllinien in Abwesenheit von Serum ernährt. Es wird auch weitverbreitet in Fusionsprotokollen und beim Wachstum von Hybridzellen eingesetzt. Dieses Medium eignet sich für die Kultivierung von normalen und neoplastischen humanen Leukozyten.

Anwendung

RPMI-1640-Medium wird als Medium zur Erhaltung von Zelllinien verwendet und dient für verschiedene Zellen als Medium.[1][2]

Sonstige Hinweise

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
RNBM1129
RNBM1128
RNBM0239
RNBM0243
RNBM0103

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2 Reviews This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. I bought this product and realized that it does not contain Hepes. Can I add hepes?

    1 answer

    1. This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.

      Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf

      Helpful?

  2. Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?

    1 answer

    1. The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

  3. buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias

    1 answer

    1. Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf

      Helpful?

  4. Can Product R8758, RPMI-1640 Medium, be frozen?

    1 answer

    1. We do not recommend freezing cell culture media. A precipitate may form upon thawing.  Also, this can cause a loss of amino acids, which may yield low cell growth.

      Helpful?

  5. Does Product R8758, RPMI-1640 Medium, contain HEPES?

    1 answer

    1. There is no HEPES in this formulation of RPMI.  We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

      Helpful?

  6. Does Product R8758, RPMI-1640 Medium, contain phenol red?

    1 answer

    1. This media contains phenol red.  The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

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  7. Does Product R8758, RPMI-1640 Medium, contain glucose?

    1 answer

    1. RPMI contains glucose at 2 g/L.  The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.

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  8. Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?

    1 answer

    1. This formulation of RPMI already contains L-glutamine at 0.3 g/L.  It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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