Heat inactivation is required based on the specific application or protocol. It is typically necessary for hybridoma cultures or immunological studies that require the inactivation of complement proteins. However, heat inactivation is not required for all applications.
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F2442
Fetales Kälberserum
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Synonym(e):
FBS, FCS, Seren, Serum
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Über diesen Artikel
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
Fetales Kälberserum (FBS) ist eine komplexe Mischung aus Biomolekülen mit geringer und hoher Molekülmasse, die optimale wachstumsfördernde und wachstumsunterdrückende Funktionen aufweist. FBS enthält Moleküle wie Wachstumsfaktoren, Proteine, Vitamine, Spurenelemente, Hormone usw. Diese Moleküle sind wichtig für das Wachstum und die Erhaltung von Zellen. Es ist eine häufig verwendete Ergänzung für Zellkulturmedien. FBS wird aus Kälberblut gewonnen. Es ist frei von Fibrin und Gerinnungsfaktoren. Die Hitzeinaktivierung von FBS blockiert das Komplementsystem. FBS aus geronnenem Blut ist die am häufigsten verwendete undefinierte Ergänzung in eukaryotischen Zellkulturen, insbesondere von Säugetieren. Es wird in der Regel mit den klassischen Medien verwendet, die in den 1950ern und 1960ern entwickelt wurden. Dazu zählen viele gängige Medien wie Minimal Essential Medium (MEM), Dulbecco′s Modified Eagle‘s Medien (DME, DMEM), Iscove′s Modified Eagle‘s Medium (IMDM), M199 und Roswell Park Memorial Institute Medium, RPMI-1640.
Application
Biochem/physiol Actions
FBS bleibt eine beliebte Medienergänzung, da es ein breites Spektrum von Funktionen in Zellkulturen bereitstellt. FBS liefert Nähstoffe, Wachstums- und Bindungsfaktoren und schützt die Zellen durch Mechanismen, die in serumfreien Medien(SFM)-Systemen schwer zu reproduzieren sind, vor oxidativen Schäden und Apoptose.
Analysis Note
- Auf Bakterien, Viren und Mykoplasmen getestet
- Unter aseptischen Bedingungen dreifach durch eine 0,1-μm-Membran filtriert.
- Endotoxin- und Hämoglobin-getestet
Other Notes
Auf das Vorhandensein von Bakterien, Viren, Mykoplasma, Endotoxine und auf den Hämoglobingehalt geprüft. Unter aseptischen Bedingungen dreifach durch eine 0,1-μm-Membran filtriert.
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Dieser Artikel | |||
|---|---|---|---|
| description USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma | description USA origin, suitable for cell culture | description USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma | description non-USA origin, γ-irradiated, sterile-filtered, suitable for cell culture |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility γ-irradiated, sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| origin USA origin | origin USA origin | origin USA origin | origin non-USA origin |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤25 mg/dL |
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Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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is heat inactivation necessary?
1 answer-
Helpful?
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How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)
1 answer-
Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.
Here's a protocol for heat inactivation:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.Helpful?
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is this Heat Inactivate FBS?
1 answer-
This product has not been heat inactivated.
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can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?
1 answer-
This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317Helpful?
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Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks
1 answer-
Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/Helpful?
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
1 answer-
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
1 answer-
FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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Are all Fetal Bovine Serum (FBS) products heat inactivated?
1 answer-
Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
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There is a precipitate in my serum. Is my serum contaminated?
1 answer-
Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.
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