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Merck

L5667

Sigma-Aldrich

LB-Agar-Ampicillin-100 Platten

pre-poured LB agar plates with 100 μg/ml ampicillin

Synonym(e):

ampicillin agar plates

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
41121800
Sterilität:
sterile; autoclaved
Eignung:
selective for Escherichia coli
selective for coliforms
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Qualität

for molecular biology

Qualitätsniveau

Sterilität

sterile; autoclaved

Form

plates (10 cm)
plates (10cm, 20-25ml each)

Verfügbarkeit

available only in USA

Kapazität

20-25 mL

Lagertemp.

2-8°C

Eignung

selective for Escherichia coli
selective for coliforms

Allgemeine Beschreibung

LB-Agar Platten mit 100 μg/ml Ampicillin
1.0% Trypton, 0.5% Hefeextrakt, 1.0% NaCl, 1.5% Agar
10 eingeschweisste Platten pro Einheit, 20-25 ml pro Platte

Anwendung

LB Agar Ampicillin-100, Plates has been used to plate and incubate the mixture of thawed bacteria and plasmid solution for vimentin expression.[1] It has also been used in the generation of the Trypanosoma brucei RNAi library.[2]

Leistungsmerkmale und Vorteile

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Komponenten

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
100μg/ml ampicillin

Sonstige Hinweise

The plates must be used within three months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Linfeng Xu et al.
Nature communications, 12(1), 6803-6803 (2021-11-25)
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation.
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes
Glover L, et al.
Nature Protocols, 10(1), 106-106 (2015)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science advances, 4(6) (2018)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science Advances, 4(6) (2018)
Daniel Del Valle Morales et al.
Bio-protocol, 10(20), e3791-e3791 (2021-03-05)
The 5' cap is a ubiquitous feature of eukaryotic mRNAs. It is added in the nucleus onto newly synthesized pre-mRNA, and in the cytoplasm onto mRNAs after decapping or endonuclease cleavage. Cytoplasmic recapping can occur after loss of the cap

Artikel

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Questions

1–2 of 2 Questions  
  1. Why do the LB-agar plates with antibiotics have such a short expiration date?

    1 answer
    1. The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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