The formulation for L3022 has not changed over the years. The "low salt" label was added later to help customers identify the formulation more easily.
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
250 g | Warenkorb auf Verfügbarkeit prüfen | € 43,70 |
1 kg | Warenkorb auf Verfügbarkeit prüfen | € 157,00 |
6 x 1 kg | Warenkorb auf Verfügbarkeit prüfen | € 864,00 |
Über diesen Artikel
grade
Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Application
Sie wird verwendet für die Resuspension von T. pyriformis-Zellfraktionen in einer Studie zur Trimastix-Fraktionierung von Zellextrakten.[1]
Features and Benefits
- Praktische, kleine Packung macht Wiegen unnötig
- Einfaches Hochskalieren durch Verwenden größerer Packungsgrößen
- Kostengünstige Alternative zu Flüssigkeiten
- Standardmäßige Formulierung
Preparation Note
2. Bei 121 °C 15 Minuten lang autoklavieren.
Zur Zubereitung der Lennox-L-Bouillon: 1 g Glucose hinzufügen und mit den Zubereitungsanweisungen wie oben fortfahren.
Zur Zubereitung des Mediums nach Enquist und Sternberg: Nach dem Autoklavieren 10 mL steriles Magnesiumsulfat 1 M unter aseptischen Bedingungen hinzufügen.
Legal Information
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Dieser Artikel | |||
|---|---|---|---|
| description Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture. | description EZMix powder microbial growth medium | description Tablet microbial growth medium | description Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture. |
| form powder | form powder | form tablet | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| composition NaCl, 5 g/L , Yeast Extract, 5 g/L , Tryptone, 10 g/L | composition - | composition - | composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
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Lagerklasse
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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Are the new batches of the product labeled as "Low salt" consistent with the older batch that does not have the same label? Consistency in experiments is a concern.
1 answer-
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the difference among the many LB - Lennox products?
1 answer-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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