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L5542
LB-Agar-Platten
pre-poured LB agar plates
Synonym(e):
pre-poured LB agar plates
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Über diesen Artikel
NACRES:
NA.74
UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
nonselective for Escherichia coli, nonselective for coliforms
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Segment
sterility
sterile; autoclaved
form
plates (10 cm), plates (10cm, 20-25ml each)
capacity
20-25 mL
storage temp.
2-8°C
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
LB Agar is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
LB Agar Plates has been used:
- to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus
- to culture numerous bacteria and is used in strain storage method
- to analyse the egestion of midgut microbes during sand fly bites
Features and Benefits
LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation
Other Notes
1.0% Tryptone
0.5% Yeast Extract
0.5% Sodium Chloride
1.5% Agar
0.5% Yeast Extract
0.5% Sodium Chloride
1.5% Agar
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Dieser Artikel | ||
|---|---|---|
| description pre-poured LB agar plates | description suitable for microbiology, NutriSelect® Plus | description Microbiologically tested |
| sterility sterile; autoclaved | sterility non-sterile | sterility - |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for bacteria (General Media), nonselective for Staphylococcus spp., nonselective for Clostridium spp., nonselective for Streptococcus spp., nonselective for coliforms, nonselective for Neisseria spp., nonselective for Escherichia coli | suitability - |
| capacity 20-25 mL | capacity - | capacity - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form plates (10 cm), plates (10cm, 20-25ml each) | form powder | form - |
| storage temp. 2-8°C | storage temp. - | storage temp. - |
Lagerklasse
13 - Non Combustible Solids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the many LB - Lennox products?
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LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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