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C2743

Cytochalasin B aus Drechslera dematioidea

Ready Made Solution, 10 mg/mL in DMSO

Synonym(e):

Phomin

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Über diesen Artikel

Empirische Formel (Hill-System):
C29H37NO5
CAS-Nummer:
Molekulargewicht:
479.61
UNSPSC Code:
51102829
NACRES:
NA.85
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1096207
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Quality Segment

form

ready-to-use solution

concentration

10 mg/mL in DMSO

antibiotic activity spectrum

fungi

application(s)

advanced drug delivery

mode of action

cell membrane | interferes

storage temp.

−20°C

SMILES string

C[C@@H]1CCC[C@@H](O)\C=C\C(=O)O[C@]23[C@@H](\C=C\C1)[C@H](O)C(=C)[C@@H](C)[C@H]2[C@H](Cc4ccccc4)NC3=O

InChI

1S/C29H37NO5/c1-18-9-7-13-22(31)15-16-25(32)35-29-23(14-8-10-18)27(33)20(3)19(2)26(29)24(30-28(29)34)17-21-11-5-4-6-12-21/h4-6,8,11-12,14-16,18-19,22-24,26-27,31,33H,3,7,9-10,13,17H2,1-2H3,(H,30,34)/b14-8+,16-15+/t18-,19-,22-,23+,24+,26+,27-,29-/m1/s1

InChI key

GBOGMAARMMDZGR-TYHYBEHESA-N

Application

Cytochalasin B is used to inhibit sugar transport in fibroblasts [1], decrease cytoskeletal actin and myosin in rabbit neutrophils [2], and to stimulate B lymphocytes [3].
Cytochalasin B from Drechslera dematioidea has been used:
  • to block cytokinesis, to obtain binucleated cells[4]
  • as a specific glucose transporter inhibitor
  • to inhibit glucose transport by class I and III GLUTs to determine the 50% inhibitory concentration (IC50) in HCMV-infected and mock-infected cells

Biochem/physiol Actions

Gehört zu einer Gruppe von Stoffwechselprodukten aus Pilzen, die eine Vielzahl zellulärer Bewegungen stören. Geeignetes Hilfsmittel für die Charakterisierung von einigen der Polymerisierungseigenschaften von Aktin und in Studien zur Zytokinese. Sonde für die beiden Hexose-Transportsysteme in L6-Myoblasten der Ratte.
Cell permeable fungal toxin that disrupts contractile microfilaments by inhibiting actin polymerization. This, in turn, induces DNA fragmentation, inhibits cell division, and disrupts many cell processes. Inhibits glucose transport.
Cytochalasin B can prevent cytoplasmic division without affecting nuclear division.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

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Dieser Artikel
C2618C6762B5936
mode of action

cell membrane | interferes

mode of action

enzyme | interferes

mode of action

DNA synthesis | interferes

mode of action

cell membrane | interferes

antibiotic activity spectrum

fungi

antibiotic activity spectrum

fungi

antibiotic activity spectrum

fungi

antibiotic activity spectrum

neoplastics

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

form

ready-to-use solution

form

ready-to-use solution

form

powder

form

ready-to-use solution

application(s)

advanced drug delivery

application(s)

advanced drug delivery

application(s)

-

application(s)

advanced drug delivery

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C


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pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 4 Oral

Lagerklasse

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

185.0 °F - closed cup - Solvent

flash_point_c

85 °C - closed cup - Solvent

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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Questions

1–4 of 4 Questions  
  1. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. Is Product C2743, Cytochalasin B, cell culture grade?

    1 answer
    1. The product has been sterile filtered, but it has not been cell culture tested by Sigma.

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  4. I have a solution of Product C2743, Cytochalasin B, in DMSO. Is the DMSO toxic to cultured cells?

    1 answer
    1. At a low enough concentration, the DMSO should not hurt the cells. The final concentration of DMSO in the aqueous working medium typically should not exceed 0.1% because higher DMSO concentrations can adversely affect many cultured cells. Dilute the product in the appropriate aqueous medium to provide a physiologically acceptable final concentration.

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