T6763

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

• Synonym(s): Acetyltrypsin
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.4.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00082094
• Specific activity: ≥8,500 BAEE units/mg protein (biuret)
• Biological source: bovine pancreas

Properties

• biological source: bovine pancreas
• Quality Segment: 200
• type: Type V-S
• form: powder
• specific activity: ≥8,500 BAEE units/mg protein (biuret)
• UniProt accession no.: Q29463
• storage temp.: −20°C
• Gene Information: cow ... TRYP8(282603)

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. [1] Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter. [2]

Biochem/physiol Actions

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Preparation Note

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Other Notes

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves