T4174

Trypsin-EDTA solution

10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

• Synonym(s): Cocoonase, Tryptar, Tryptase
• EC Number: 3.4.21.4 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.75
• MDL number: MFCD00130286
• Biological source: Porcine
• Concentration: 10 ×

Properties

• biological source: Porcine
• Quality Segment: 400
• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• mol wt: 23.4 kDa
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: Porcine parvovirus, none detected (9 CFR)
• pH: 7.0-7.6
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
Trypsin-EDTA solution is used for the following applications:

• Used as a supplement in cell culture for their maintenance[1]
• In harvesting cells grown to confluence[2]
• to detach lentivirus-transduced macrophages[3]

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Preparation Note

Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution contaiing no Ca²⁺ or Mg²⁺.

Other Notes

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Disclaimer

This product is stored frozen between -10 and -40°C. Repeated cycles of freezing and thawing should be avoided.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable