QPBCA

QuantiPro^™ BCA Assay Kit

for 0.5-30 μg/ml protein

• Synonym(s): BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
• NACRES: NA.84
• UNSPSC Code: 12161503

Properties

• Quality Segment: 200
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

In addition to protein determination and quantification in solution, the QuantiPro^™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.

Biochem/physiol Actions

Based on the alkaline reduction of Cu²⁺ to Cu¹⁺ by proteins, and the formation of a bicinchoninic acid:Cu¹⁺ complex having an absorbance maximum at 562 nm.

Features and Benefits

• Accurate across a broad range of protein concentrations
• High sensitivity; linear response form 0.5 to 30 μg/ml of protein
• Stable color complex
• Reduced susceptibility to detergents

Preparation Note

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Legal Information

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Kit Components Also Available Separately

• P0914

  Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1

  SDS

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H290,H315,H318,H410
• pcodes: P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class: 10 - Combustible liquids