P9290

Proteinase K–Agarose from Tritirachium album

lyophilized powder

• Synonym(s): Proteinase K-Agarose
• UNSPSC Code: 41106500
• NACRES: NA.56
• MDL number: MFCD00132131
• Form: lyophilized powder
• Biological source: microbial (T.album; T. ALBUM)

Properties

• biological source: microbial (T.album; T. ALBUM)
• Quality Segment: 200
• form: lyophilized powder
• extent of labeling: 30-140 units per g solid
• technique(s): DNA extraction: suitable, RNA extraction: suitable, protein extraction: suitable
• swelling: 1 g swells to ~5 mL
• storage temp.: −20°C

Description

Application

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

Biochem/physiol Actions

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

Features and Benefits

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

Physical form

Lyophilized powder stabilized with lactose

Other Notes

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable