Elemental analysis of this material has not been performed. The sulfate content is not determined.
P8169
Phytagel™
BioReagent, suitable for plant cell culture, powder
Phytagel™
Synonym(s):
Gellan Gum, Agar substitute gelling agent
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
Pricing and availability is not currently available.
product line
BioReagent
Quality Segment
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
General description
Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.
Application
Phytagel™ has been used:
- in the solidification of culture medium for root assays in Arabidopsis thaliana
- as a component of Solid Paul′s medium for Agrobacterium culture
- as a component of root induction medium in Ilex dumosa tissue culture
Biochem/physiol Actions
Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
Preparation Note
To prevent clumping, add slowly to rapidly stirring medium prior to heating.
Other Notes
An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
Legal Information
Phytagel is a trademark of Sigma-Aldrich Co. LLC
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| form powder | form - | form powder | form powder |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Instructions
Christine Le Roux et al.
Applied and environmental microbiology, 75(24), 7752-7759 (2009-10-27)
In the context of an increasing utilization of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree in the tropical humid zone, its symbiotic characterization was carried out in comparison with that of its two parental species.
Yasuka L Yamaguchi et al.
Frontiers in plant science, 8, 1195-1195 (2017-07-28)
Developmental plasticity is one of the most striking features of plant morphogenesis, as plants are able to vary their shapes in response to environmental cues. Biotic or abiotic stimuli often promote organogenesis events in plants not observed under normal growth
Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells
Buschmann H, et al.
The New phytologist, 190(1), 258-267 (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8169-100G | 04061835569274 |
| P8169-250G | 04061835569281 |
| P8169-5KG | 04061835569304 |
| P8169-500G | 04061835569298 |
| P8169-1KG | 04061835442683 |
-
What is the sulfate content, if any, of Phytagel?
1 answer-
Helpful?
-
-
Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione
1 answer-
This product is characterized as having a low acyl content.
Helpful?
-
-
What is the molecular weight of one repeating unit?
1 answer-
The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.
Helpful?
-
-
What is the gelling strength of Phytagel P8169
1 answer-
The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).
Helpful?
-