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P5381
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)
Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
Synonym(s):
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-603-1
MDL number:
Specific activity:
≥400 units/mg protein (biuret)
Biological source:
bakers yeast
Concentration:
≥3 mg/mL protein Biuret
Pricing and availability is not currently available.
biological source
bakers yeast
Quality Segment
type
Type III
form
ammonium sulfate suspension
specific activity
≥400 units/mg protein (biuret)
mol wt
tetramer 119,500 Da±600
concentration
≥3 mg/mL protein Biuret
technique(s)
activity assay: suitable
color
white
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
yeast ... PGI1(852495)
General description
Research area: Cell Signaling
Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.[1]
Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.[1]
Application
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
- to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.[2]
- as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
- to convert fructose to glucose while evaluating fructose content in kudzu roots.
Biochem/physiol Actions
Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP).[3] It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.[3]
Physical form
Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0
Other Notes
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.
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| Gene Information yeast ... PGI1(852495) | Gene Information - | Gene Information - | Gene Information - |
| technique(s) activity assay: suitable | technique(s) photometry: suitable | technique(s) - | technique(s) - |
| biological source bakers yeast | biological source - | biological source - | biological source bakers yeast |
| specific activity ≥400 units/mg protein (biuret) | specific activity - | specific activity ~10,000 units/mg protein | specific activity ≥40 units/mg protein |
| concentration ≥3 mg/mL protein Biuret | concentration - | concentration - | concentration - |
| form ammonium sulfate suspension | form - | form ammonium sulfate suspension | form lyophilized powder |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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QC Methods
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P5381-5KU | 04061834379362 |
| P5381-1KU | 04061834379294 |
| P5381-50KU | 04061834379348 |
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What is the Department of Transportation shipping information for this product?
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What is the pH optimum of Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?
1 answer-
The pH optimum for yeast PGI (Product No. P5381) is 7, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).
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What is the substrate for Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?
1 answer-
The substrate for PGI is D-fructose-6-phosphate, according to our Enzyme Assay procedure.
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What is the KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate, and what does that mean?
1 answer-
KM is the concentration of substrate that leads to half-maximal velocity. The KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate is 0.167, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).
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What is the typical protein concentration for Product No. P5381 (PGI)?
1 answer-
The protein concentration of Product No. P5381 (PGI) can vary from lot-to-lot. It is typically in the range of 5-20 mg protein per mL. The lot-specific concentration will be given on the certificate of analysis.
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