Based on the available product and technical documentation, there is no explicit information regarding the inactivation (including heat inactivation), molecular weight, or filtration of Phosphodiesterase I (P3243) from Crotalus adamanteus venom
P3243
Phosphodiesterase I from Crotalus adamanteus venom
vial of ≥100 units, Purified
Synonym(s):
5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-806-5
MDL number:
Specific activity:
≥20.0 unit/mg solid
Biological source:
Crotalus adamanteus venom
Pricing and availability is not currently available.
biological source
Crotalus adamanteus venom
Quality Level
form
solid
quality
Purified
specific activity
≥20.0 unit/mg solid
packaging
vial of ≥100 units
storage temp.
−20°C
Application
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides[1].
Biochem/physiol Actions
Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.
Preparation Note
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
Purified via the method of Williams, et al.[2] and further treated to inactivate contaminating 5′-nucleotidase activity.
Other Notes
One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥20.0 unit/mg solid | specific activity - | specific activity - | specific activity ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase) |
| biological source Crotalus adamanteus venom | biological source - | biological source - | biological source Penicillium citrinum |
| form solid | form crude dried venom | form crude dried venom | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging vial of ≥100 units | packaging - | packaging - | packaging vial of ≥250 units (using RNA substrate) |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Valérie de Crécy-Lagard et al.
Molecular biology and evolution, 27(9), 2062-2077 (2010-04-13)
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA(Phe)), 3' adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a
Action of venom phosphodiesterase on deoxyribonucleic acid.
E J WILLIAMS et al.
The Journal of biological chemistry, 236, 1130-1134 (1961-04-01)
N Shenoy et al.
Blood cancer journal, 7(7), e587-e587 (2017-07-22)
The Ten Eleven Translocation (TET) enzymes have been found to be mutated in both diffuse large B-cell (DLBCL) and peripheral T-cell (PTCL) lymphomas resulting in DNA hypermethylation. Recent studies in embryonal stem cells showed that ascorbic acid (AA) is a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3243-1VL | 04061834367529 |
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How can I inactivate the PDE before adding it on my cell culture ? Heat inactivation ? And what is the MW of this PDE ? can it be filtrated ? Thanks in advance
1 answer-
Helpful?
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1. If I want to dissolve this venom what I can use distilled water or any buffer? And what is the storage temperature after reconstitution? 2. If I want to increase the efficiency of venom what I can use?
1 answer-
For testing purposes, this product is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. The solution stability and storage conditions have not been determined. The enzyme may also be dissolved in 0.11 M Tris HCl buffer, pH 8.9 with 0.11 M NaCl and 15 mM MgCl2 according to Worthington Manual, p. 310 (1993). Note that this material is not the raw venom, but the purified enzyme. The enzyme is purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.
Helpful?
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What buffer can be used to Store PDE I, and is it soluble in water?
1 answer-
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. The solution stability has not been determined.
Helpful?
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