To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Synonym(s):
Alkaline phosphatase-Agarose
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About This Item
Specific activity:
250-1000 units/mL packed gel
Biological source:
bovine (calf) intestine
Pricing and availability is not currently available.
biological source
bovine (calf) intestine
Quality Level
form
ammonium sulfate suspension
specific activity
250-1000 units/mL packed gel
matrix
beaded agarose
storage temp.
2-8°C
General description
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
Application
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases.
Biochem/physiol Actions
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Packaging
Package size based on DEA units
Physical form
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Other Notes
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
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This Item | |||
|---|---|---|---|
| biological source bovine (calf) intestine | biological source bovine (calf) intestine | biological source bovine (calf) intestine | biological source bovine intestinal mucosa |
| specific activity 250-1000 units/mL packed gel | specific activity ≥1500 U/mg | specific activity ~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer) | specific activity ≥2,000 DEA units/mg protein |
| form ammonium sulfate suspension | form liquid | form solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 200 |
| matrix beaded agarose | matrix - | matrix - | matrix - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Li-Na Li et al.
Guang pu xue yu guang pu fen xi = Guang pu, 29(10), 2820-2823 (2009-12-30)
To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
Rebika Shrestha et al.
iScience, 25(1), 103608-103608 (2022-02-03)
Understanding the spatiotemporal distribution and dynamics of RAS on the plasma membrane (PM) is the key for elucidating the molecular mechanisms of the RAS signaling pathway. Single particle tracking (SPT) experiments show that in cells, KRAS diffuses in at least
J E Coleman
Annual review of biophysics and biomolecular structure, 21, 441-483 (1992-01-01)
Alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two Zn ions and one Mg ion) are present at the active center. Zn ions at all three sites also produce a maximally
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P0762-1KU | 04061834353027 |
| P0762-250UN | 04061834353034 |
| P0762-100UN | 04061832758664 |
| P0762-50UN | 04061834353041 |
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1. What is the recommended speed for spinning down the phosphatase, alkaline-agarose when using this product?
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