The purified Mouse IgG is isolated from pooled normal mouse serum by fractionation and ion-exchange chromatography.
I5381
IgG from mouse serum
reagent grade, ≥95% (SDS-PAGE), lyophilized powder
Synonym(s):
Mouse IgG
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About This Item
Conjugate:
unconjugated
Species reactivity:
-
Application:
—
Citations:
259
Pricing and availability is not currently available.
conjugate
unconjugated
Quality Segment
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
lyophilized powder
composition
Protein, ≥80%
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[1]
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.
Application
Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA[2] and chromatin immunoprecipitation assay.[3] It was used to coat nano particles in the development of biosensors.[4]
Physical form
Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| form lyophilized powder | form buffered aqueous solution | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| grade reagent grade | grade technical grade | grade reagent grade | grade reagent grade |
| assay ≥95% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| target post-translational modification unmodified | target post-translational modification - | target post-translational modification unmodified | target post-translational modification unmodified |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is I5381 from one mouse or several mice?
1 answer-
Helpful?
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Bonjr,Pouvez vous svp me confirmer que la poudre ne contient pas de conservateur (type sodium azide) et que ces igG sont une fois reconstitués suitable pour cell culture ? Est ce que la solution de reconstitution est fournie avec la poudre à part ? Merci
1 answer-
Product I5381 is azide-free. There is no addition or treatment with sodium azide during the lyophilization process.
Helpful?
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