Gamborg's B-5 Basal Medium with Minimal Organics is a plant tissue culture medium that contains reduced amounts of organic compounds compared to the standard Gamborg's B-5 Basal Medium. The minimal organics version is designed to provide a lower concentration of organic compounds, such as vitamins or amino acids, which allows for more precise control over nutrient supplementation in plant tissue culture experiments. Please see the link below to review the formulation tables for Gamborg's basal salt mixtures, products G5893 and G5768.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/186/351/g5768for.pdf
G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Gamborg’s B-5 Basal Medium with Minimal Organics
Synonym(s):
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
NACRES:
NA.72
UNSPSC Code:
12352207
Pricing and availability is not currently available.
form
fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings[1]
- as a component of solid GM medium for germination of Arabidopsis seeds[2]
Preparation Note
Formulated to contain 3.2 grams of powder per liter of medium.
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
1 of 1
This Item | |||
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| form fine powder | form fine powder | form powder | form powder |
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Warning
hcodes
Hazard Classifications
Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
Martin E Kolewe et al.
Plant cell reports, 29(5), 485-494 (2010-03-11)
Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G5893-1L | 04061833638675 |
| G5893-10L | 04061833638668 |
-
What do you mean by minimal organics?
1 answer-
Helpful?
-