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G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, >= 550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
EC Number:
Specific activity:
>= 550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
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biological source

bacterial (Leuconostoc mesenteroides)

Quality Segment

type

Type XXIV

form

lyophilized powder

specific activity

>= 550 units/mg protein (biuret)

mol wt

128 kDa

composition

Protein, 15-40% biuret

application(s)

agriculture

foreign activity

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%

storage temp.

2-8°C

General description

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer.[1] Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.[2]

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.[3][4][5]

Biochem/physiol Actions

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism.[2]
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

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1 of 1

This Item
G5760G4134G8529
biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

-

biological source

-

specific activity

>= 550 units/mg protein (biuret)

specific activity

550-1,100 units/mg protein (biuret)

specific activity

200-400 units/mg protein (modified Warburg-Christian)

specific activity

≥550 units/mg protein (biuret)

application(s)

agriculture

application(s)

-

application(s)

agriculture

application(s)

agriculture

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized powder

mol wt

128 kDa

mol wt

54 kDa

mol wt

128 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Related Content

Product Information Sheet






Global Trade Item Number

SKUGTIN
G5885-2KU04061833638620
G5885-1KU04061833638606
G5885-10KU04061832729893
G5885-200UN04061833638613

Questions

1–5 of 5 Questions  
  1. What is the molecular weight of Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    1 answer
    1. The molecular weight of this enzyme has not been determined.  However, Biochem. Biophys. Res. Comm. 59, 894 (1974) reports a molecular weight of 55,000 daltons for the monomer.  The enzyme is functionally active as a dimer or tetramer.

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  2. What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    1 answer
    1. Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. In which solvent can Product No. G5885, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?

    1 answer
    1. The product can be dissolved at 1 mg/ml in 5 mM glycine buffer , pH 8, (with or without 0.1% BSA)or in deionized water at 1 mg/ml (buffer is preferable).

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  5. Can solutions of Product No. G5885, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc  mesenteroides, be stored?

    1 answer
    1. Solutions in buffer may be aliquotted and stored frozen at -20°C for about 1-2 months.

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