D3687

DirectLoad^™ PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis

• Synonym(s): 100bp ladder for gel electrophoresis, 100bp maker, DirectLoad^™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker
• NACRES: NA.31
• UNSPSC Code: 41105335
• Form: liquid
• Solubility: water: soluble
• Storage temp.: −20°C

Properties

• grade: Molecular Biology
• Quality Level: 200
• form: liquid
• usage: 75 uses
• solubility: water: soluble
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s DirectLoad^™ PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sigma′s DirectLoad^™ PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable