D0672
φX174 DNA Marker Hae III Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Storage temp.:
−20°C
Pricing and availability is not currently available.
General description
Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Preparation Note
φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
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| Quality Level 100, 200 | Quality Level 100, 200 | Quality Level 100, 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| usage 200 uses (0.1 mg size), 40 uses (20 μg size) | usage 200 uses (0.1 mg size), 40 uses (20 μg size) | usage 100 uses (0.1 mg size), 500 uses (0.5 mg size) | usage 100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size) |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D0672-.1MG | 04061833557969 |
| D0672-20UG | 04061835572465 |