A3422

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

• Synonym(s): ACP, Achromopeptidase
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.4.21.50 (BRENDA, IUBMB)
• MDL number: MFCD00130333
• Specific activity: ≥20,000 units/mg solid

Properties

• form: partially purified powder
• Quality Segment: 200
• specific activity: ≥20,000 units/mg solid
• mol wt: 27 kDa
• foreign activity: Collagenase, present
• storage temp.: −20°C

Description

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Achromopeptidase from bacteria may be used:

• in the homogenization step for genomic DNA extraction from microbial biofilm
• in the lysis of gram-positive strains(
• for DNA extraction from Nocardia strains

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Physical form

Partially purified powder.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves