The exact value will be listed on the lot specific Certificate of Analysis. A Certificate can be accessed in the DOCUMENTATION section under 'Certificate of Analysis'. Please see the link below to access a specific lot or sample Certificate:
https://www.sigmaaldrich.com/product/sigma/a1153#product-documentation
A1153
Aprotinin
3-8 TIU/mg solid, lyophilized powder
Synonym(s):
BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
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About This Item
Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
Molecular Weight:
6511.44
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-994-9
MDL number:
eCl@ss:
32190102
Pricing and availability is not currently available.
Product Name
Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid
biological source
bovine lung
Quality Level
form
lyophilized powder
specific activity
3-8 TIU/mg solid
mol wt
~6,500
solubility
H2O: ≥5 mg/mL
UniProt accession no.
storage temp.
2-8°C
SMILES string
S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O
InChI
1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(41
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
Gene Information
cow ... PTI(404172)
General description
Aprotinin from bovine lung is a globular polypeptide monomer with a molecular weight of 6.5 kDa. Commonly used as a non-specific serine protease inhibitor, aprotinin contains an antiparallel β sheet, N-terminal 310 helix and C-terminal and α helix. Aprotinin residues from amino acids 13 - 18 are essential for binding to serine proteases.[1]
Application
Aprotinin is largely used as an inhibitor of trypsin.
Aprotinin from bovine lung has been used:
- as a protease inhibitor in radioimmunoprecipitation assay buffer (RIPA) for the homogenization of cardiac microvascular endothelial cells (CMECs)(4) and mammary epithelial cells
- in angiogenesis assay for fibroblast
- in the proteomic stabilization of saliva supernatant
Biochem/physiol Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Aprotinin inhibits proteases like trypsin, plasmin, chymotrypsin and thrombin. It blocks the bradykinin synthesis from kininogen. It is useful for treating blood loss during surgery.[1]
Preparation Note
This product is a lyophilized powder with activity of 3-8 TIU/mg of solid powder. Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions tend to be less stable than concentrated ones, though solution stability also depends on pH. Aprotinin is relatively stable against denaturation - only thermolysin has been found capable of degrading it at 60-80°C. Sterilizaiton of Aprotinin can be achieved through filtration by a 0.2 μm filter.
Other Notes
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine lung | biological source bovine lung | biological source bovine lung | biological source bovine lung |
| Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| solubility H2O: ≥5 mg/mL | solubility H2O: >10 mg/mL | solubility H2O: 5 mg/mL | solubility glycerol: 3 mg/mL, clear, colorless (equilibration buffer containing 5% glycerol) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
RNAPro? SAL: A device for rapid and standardized collection of saliva RNA and proteins
Chiang SH, et al.
Biotechniques, 58(2), 69-76 (2015)
Aprotinin interacts with substrate-binding site of human dipeptidyl peptidase III
Agic D, et al.
Journal of Biomolecular Structure & Dynamics, 8(1), 1-11 (2019)
Perioperative systemic haemostatic agents
Mahdy AM and Webster NR
British journal of anaesthesia, 93(6), 842-858 (2004)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A1153-250MG | 04061835511273 |
| A1153-100MG | 04061835551071 |
| A1153-25MG | 04061835511280 |
| A1153-1MG | 04061835551088 |
| A1153-10MG | 04061835511266 |
| A1153-5MG | 04061835540501 |
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What is the exact TIU/mg for the product? The information gives a range (3-8TIU/mg) but I need the exact value in order to calculate what size I need to order based on my samples.
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Aprotinin from bovine lung, Product A1153, the same as Trasylol?
1 answer-
Trasylol is the Bayer AG registered trademark name for this material.
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What type of inhibitor is Aprotinin from bovine lung, Product A1153?
1 answer-
Aprotinin is a competitive serine protease inhibitor which forms stable complexes that block the active site of the enzyme.
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When using Product A1153, Aprotinin from bovine lung, what is the conversion from KIU to TIU?
1 answer-
According to data from our labs here at Sigma, there are approximately 1300 KIU per 1 TIU. This, along with other useful information, can be found on the product information sheet available at our website: A1153 Product Information Sheet
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What are typical concentrations of Aprotinin from bovine lung, Product A1153, to use as a protease inhibitor?
1 answer-
Typical concentration of Aprotinin to be used as a protease inhibitor is 1 μM or approximately 6.5 μg/ml.
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How should I store solutions of Aprotinin from bovine lung, Product A1153?
1 answer-
Sterile solutions of aprotinin may be stored at 4 °C or as frozen aliquots at -20 °C.
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