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93017

SSC Buffer 20x

for Northern and Southern blotting, solution

Synonym(s):

20x SSC

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700
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grade

Molecular Biology

Quality Segment

product line

BioReagent

form

solution

technique(s)

Southern blotting: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

7.0±0.2

density

1.16 g/mL at 20 °C

storage temp.

room temp

General description

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Preparation Note

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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This Item
S2015S6639S0902
technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

-

product line

-

form

solution

form

solution

form

solution

form

powder blend

pH

7.0±0.2

pH

-

pH

6.9 —7.1

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Global Trade Item Number

SKUGTIN
93017-10L-F04061833271360

Questions

  1. Hi how long time can I keep open sscx20 solution? What the expire time for this? Same question i have for ddulitions for this bbuffer, f.eks 2xssc?

    1 answer
    1. Provided the product is handled proprerly and not contaminated, it is quite stable. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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