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4352157

Trypsin, TPCK-Treated

Synonym(s):

6- (1-tosylamido-2-phenyl) ethyl chloromethyl ketone treated Trypsin, Modified trypsin (TPCK-Treated), Trypsin, TPCK treated

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About This Item

NACRES:
NA.56
UNSPSC Code:
12352204
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shipped in

dry ice

storage temp.

−20°C

General description

Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of Lysine and Arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. This trypsin product also contains calcium chloride, which may improve consistency in digestion. Each package contains 10 vials, with 25 μg in each vial. 

Application

Trypsin, TPCK-Treated has been used in enzymatic treatment to cleave the recombinant native B-cell lymphoma-extra large (Bcl-xL)-ΔTM monomer to generate fibrils (the third type of Bcl-xL fibril, called “BclxLcf37”).[1] It has also been used in influenza infection experiments as a supplement in infection/DMEM serum-free medium to infect A549 cells with influenza A H1N1 virus at a multiplicity of infection (MOI) to perform multi-round infection.[2]
Trypsin, TPCK-Treated has been used for the digestion of protein samples to generate peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.[3][4]

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1

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Questions

1–2 of 2 Questions  

  1. What is the most suitable buffer to reconstitute it considering a protocol to activate influenza virus prior to mammalian culture infection?

    1 answer

    1. This product has been used in influenza infection experiments as a supplement in the infection medium. The medium used is DMEM serum-free medium supplemented with 50 IU/ml penicillin, 50 mg/ml streptomycin, and 0.2 mg/ml TPCK-trypsin. Relevant references include:
      "Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy," PLoS Pathogens (2021-02-18), PMID: 33596274, Irene Pila-Castellanos, Diana Molino, Joe McKellar, et al.
      "Viruses traverse the human proteome through peptide interfaces that can be biomimetically leveraged for drug discovery," L Meyniel-Schicklin, J Amaudrut… - Proceedings of the National Academy of Sciences, 2024.

      Helpful?

  2. Hi, We wonder how is the best buffer or solution to resuspend this product?. Cat 352157 Sigma-Aldrich. Trypsin, TPCK-Treated

    1 answer

    1. For the purpose of in-gel tryptic digestion, it is recommended to reconstitute a vial of trypsin with 1 mL of 100 mM ammonium bicarbonate (NH4HCO3) pH 8.5.

      Helpful?

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