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PPB006

Sigma-Aldrich

Phosphate buffered saline

powder, pH 7.4, pHast Pack™

Synonym(s):

1X PBS wash buffer, PBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12161700
NACRES:
NA.21
Pricing and availability is not currently available.

Product Name

Phosphate buffered saline, pH 7.4, pHast Pack™, powder

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry)

impurities

<5—2 ppm Heavy metals (Lead)

color

white

pH

7.4

solubility

soluble, clear, colorless

cation traces

Fe: <10 ppm

foreign activity

DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected

storage temp.

room temp

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General description

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Application

Phosphate buffered saline is commonly used in washing solutions for:
  • ELISA
  • Western Blotting and other protein applications
  • Cell culture (sterile filtration recommended)
  • Resuspension and sonication of cells
  • Immunohistochemistry

Features and Benefits

  • Save time with our ready-to-use formulation
  • No calculations, measurements, and adjusting pH
  • Reliable quality for reproducibility
  • Suitable for use in cell culture and electrophoresis
  • Biological tests: free of DNase, RNase, Protease, and Nickase
  • Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCK2951

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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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