AB758B

Anti-Collagen Type I Antibody, biotin conjugated

Chemicon^®, from goat

• Synonym(s): Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: biotin conjugate
• Clone: polyclonal
• Application: DB, ELISA, IHC, WB
• Citations: 2

Properties

• biological source: goat
• Quality Level: 100
• conjugate: biotin conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: bovine, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, western blot: suitable
• suitability: not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_000088.3
• UniProt accession no.: P02452
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... COL1A1(1277)

Description

Immunogen

Human placental collagen type I

Application

Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.

Dot and slot blotting: 1:500-1:1,000

ELISA: 1:1,000-4,000

Indirect immunohistochemistry (frozen sections only): 1:20-1:40

The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Biochem/physiol Actions

Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Physical form

Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose^™ 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable