475989

MTT

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

• Synonym(s): MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
• Empirical Formula (Hill Notation): C₁₈H₁₆BrN₅S
• CAS Number: 298-93-1
• Molecular Weight: 414.32
• UNSPSC Code: 12171500
• NACRES: NA.25
• MDL number: MFCD00011964

Properties

• Quality Level: 100
• description: RTECS - XF8060000
• assay: ≥98% (silver nitrate titration)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤0.5% Sulfated ash
• color: yellow
• solubility: PBS: 2 mg/mL
• cation traces: heavy metals: ≤0.001%
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: [s]1c(nc(c1C)C)[n]2[n+](nc(n2)c4ccccc4)c3ccccc3.[Br-]
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Description

General description

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H315,H319,H335,H341
• pcodes: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable