Key Documents

View All Documentation

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Name: Phospholipase C, Phosphatidylinositol-specific from <I>Bacillus cereus</I>
Brand: SIGMA

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

CAS Number:

37288-19-0

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-638-2

MDL number:

MFCD00131896

EC Number:

4.6.1.13 (BRENDA, IUBMB)

Specific activity:

≥1,000 units/mg protein (Lowry)

Biological source:

Bacillus sp. (Bacillus cereus)

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

Bacillus sp. (Bacillus cereus)

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

≥1,000 units/mg protein (Lowry)

mol wt

28 kDa

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

storage temp.

2-8°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Description

General description

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
to cleave immunolabeled HeLa cells

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	P5542	525186	P6621
Sigma-Aldrich P8804 Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus	Sigma-Aldrich P5542 Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus	Sigma-Aldrich 525186 Phospholipase C, Bacillus cereus	Sigma-Aldrich P6621 Phospholipase C from Bacillus cereus
Gene Information Bacillus cereus E33L ... BCZK3513(3026815)	Gene Information Bacillus cereus E33L ... BCZK3513(3026815)	Gene Information -	Gene Information Bacillus cereus ATCC 10987 ... plC(2749087)
specific activity ≥1,000 units/mg protein (Lowry)	specific activity ≥1,000 units/mg protein (Lowry)	specific activity ≥50 units/mg dry wt.	specific activity ≥200 units/mg protein
biological source Bacillus sp. (Bacillus cereus)	biological source -	biological source -	biological source -
form buffered aqueous glycerol solution	form lyophilized powder	form solid	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 28 kDa	mol wt -	mol wt -	mol wt 23-27 kDa by SDS-PAGE

Still not finding the right product?

Explore all of our products under Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000469923

0000427386

0000119053

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKU	GTIN
P8804-25UN	04061832856858
P8804-5UN	04061832856865

Questions

Reviews

No rating value