The frosted side (white side) is the the charged side.
P0425
Poly-Prep Slides
poly-L-lysine coated glass slides
Synonym(s):
Poly-L-Lysine-coated glass slides
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About This Item
NACRES:
NA.47
UNSPSC Code:
41116124
Pricing and availability is not currently available.
Quality Segment
shelf life
Expiry date on the label.
application(s)
hematology
histology
storage temp.
room temp
General description
Poly-Prep slides are made of glass and designed for cell adhesion. The slides are coated with Poly-L-Lysine, a non-specific attachment factor for cells useful in promoting cell adhesion on solid substrates. Poly-L-lysine enhances binding between the cell membrane and the cultured surface.
Application
Poly-Prep Slides are used:
- To mount liver and lung cell sections and mounted on poly-prep slides.
- To prove better cell adhesion in clinical bronchoalveolar lavage fluid samples.
- To determine pAkt (alpha serine/threonine-protein kinase) expression by immunohistochemistry.
- In immunofluorescence imaging of nuclear translocation of nuclear factor κB(NFκB) in A549 cells.
- To study the phospholipase C-zeta activity of sperm.
- To prepare coelomocytes smears, which were further subjected to antibody staining.
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| application(s) hematology | application(s) - | application(s) - | application(s) - |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. - |
| shelf life Expiry date on the label. | shelf life - | shelf life - | shelf life - |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level - |
3-aminopropyltriethoxysilane, tissue section, tissue adhesive microscope slides, poly-l-lysine, histology | - | - | - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The frosted side is the charged side?
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Is it positively charged?
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The Poly Prep slides (P0425) are positively charged.
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How many slides will be present?
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This product comes in a pack of 72 slides.
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Would you recommend wiping the slides with ethanol before use? What alternatives can be used if not ethanol? I'm seeing some artifacts in microscopy which is not from the dyes or tissue.
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Assuming the Poly-Prep Slides are clean, it is doubtful that dipping the slides into ethanol or other solvents will decrease the artifact you are seeing. Poly-Prep slides have been noted to have blue fluorescence in the UV range. There are reference articles that mention blue fluorescence of L-Lysine in the UV range. The full PDF can be downloaded at the URL provided below. In house experience with the polymer matches that of the monomer.
https://www.sciencedirect.com/science/article/pii/S1386142522008666
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What's size of the slides?
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These slides are rectangular with frosting at one edge.
The dimensions are given as 3 inches (~75mm) x 1 inch (~25mm) with a thickness of 1mm.
The coated area is approx 18 to 20 mm from one edge.Helpful?
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How can the side of the slide that is poly-L-lysine coated be differentiated?
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The coating is visible as white. Using a lead pencil to identify which side of the surface has been coated is suggested, as the lead will mark the coated side but not the uncoated glass.
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How many slides comes into the package?
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There are 72 slides in each package of this item.
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