M3003

Myokinase from rabbit muscle

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

• Synonym(s): ATP: AMP phosphotransferase, Adenylate Kinase
• CAS Number: 9013-02-9
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-743-3
• NACRES: NA.54
• MDL number: MFCD00131644
• EC Number: 2.7.4.3 (BRENDA, IUBMB)
• Specific activity: 1,500-3,000 units/mg protein (biuret)
• Biological source: rabbit muscle

Properties

• biological source: rabbit muscle
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: 1,500-3,000 units/mg protein (biuret)
• mol wt: 21 kDa
• foreign activity: 3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%
• storage temp.: 2-8°C

Description

General description

Research area: Cell Signaling
Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.

Application

Myokinase has been used:

• in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate [1]
• in a study to investigate the ligand-binding properties of its peptide fragments [2]
• as one of the components in the ATP generation solution for the quantification of 3’3’-c-di-AMP using an AMP-Luciferase assay [3]

Biochem/physiol Actions

Myokinase or adenylate kinases, play a crucial role in regulating energy metabolism within cells and balancing adenine nucleotide ratios in different cellular compartments.

Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP

Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity. [4]

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution containing 0.001 M EDTA, pH 6.0

Other Notes

One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)