M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

• Synonym(s): Moloney Murine Leukemia Virus Reverse Transcriptase
• CAS Number: 9068-38-6
• UNSPSC Code: 12352202
• NACRES: NA.55
• MDL number: MFCD00283754
• Biological source: Porcine intestinal mucosa
• Recombinant: expressed in E. coli
• Concentration: 200 units/μL

Properties

• biological source: Porcine intestinal mucosa
• Quality Segment: 200
• recombinant: expressed in E. coli
• form: liquid
• usage: sufficient for 200 reactions
• feature: dNTPs included: no, hotstart: no
• concentration: 200 units/μL
• technique(s): RT-PCR: suitable
• color: colorless
• input: purified RNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Application

M-MLV Reverse Transcriptase has been used:

• for the preparation of cDNA libraries or for first strand cDNA synthesis[1]
• for use in a 2-step RT-PCR assay[2][3]
• in quantitative realtime-polymerase chain reaction (RT-qPCR)[2]
• in reverse transcription[3]

Features and Benefits

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.

Packaging

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Other Notes

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)