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G7765

Gelatin from cold water fish skin

40-50% in H2O

Synonym(s):

Gelatine, Teleostean gelatin

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About This Item

CAS Number:
9000-70-8
MDL number:
MFCD00081638
NACRES:
NA.61
UNSPSC Code:
12352202
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biological source

fish skin

Quality Level

200

form

liquid

mol wt

~60 kDa

contains

0.15% propyl p-hydroxybenzoate as preservative, 0.2% methyl p-hydroxybenzoate as preservative

concentration

40-50% in H2O

technique(s)

ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable

storage temp.

2-8°C

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.
Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.

Application

Used as a blocking agent in immunochemistry.
Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections,[1] as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.

Biochem/physiol Actions

Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.

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This Item
48722G2500G1890
Gelatin from cold water fish skin 40-50% in H2O

Sigma-Aldrich

G7765

Gelatin from cold water fish skin

Gelatin from porcine skin medium gel strength, suitable for microbiology

Millipore

48722

Gelatin from porcine skin

Gelatin from porcine skin gel strength 300, Type A

Sigma-Aldrich

G2500

Gelatin from porcine skin

Gelatin from porcine skin powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

Sigma-Aldrich

G1890

Gelatin from porcine skin

technique(s)

ELISA: suitable, immunocytochemistry: suitable, cell culture | mammalian: suitable, western blot: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

room temp

concentration

40-50% in H2O

concentration

-

concentration

-

concentration

70—90% Biuret

mol wt

~60 kDa

mol wt

-

mol wt

-

mol wt

50—100 kDa

Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000551314
0000535723
0000527931
0000527931
0000535723

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OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress
Wehner K A, et al.
Molecular and Cellular Biology, 30(8), 2006-2016 (2010)
Soybean protein concentrate as a protein source for turbot Scophthalmus maximus L
O.J. Day and H.G. Plascencia GonzAlez
Aquaculture (Amsterdam, Netherlands), 4, 221-228 (2000)
Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues.
Freistadt M S, et al.
Molecular and Cellular Biology, 10(11), 5700-5706 (1990)
View All Related Papers

Global Trade Item Number

SKUGTIN
G7765-1L04061833643143
G7765-250ML04061833643150

Questions

1–6 of 6 Questions  

  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. What is Product G7765, Gelatin from cold water fish skin, soluble in?

    1 answer

    1. Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.

      Helpful?

  3. What is the gel point of Product G7765, Gelatin from cold water fish skin?

    1 answer

    1. The gel point of Product G7765, Gelatin from cold water fish skin is 5-10°C. The product will be a gel when storing the product at 2-8°C.

      Helpful?

  4. What is the solution stability of Product G7765, Gelatin from cold water fish skin?

    1 answer

    1. The specification for shelf life is 2 years for unopened bottles stored at 2-8°C.  Each lot is assigned a retest date; this date is given on the Certificate of Analysis avialable from our website.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the difference between type A and type B gelatin?

    1 answer

    1. Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2.

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