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FLAAB

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer

lyophilized powder

Synonym(s):

ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP

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About This Item

NACRES:
NA.84
eCl@ss:
32160414
UNSPSC Code:
41116133
Pricing and availability is not currently available.
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form

lyophilized powder

Quality Level

200

technique(s)

activity assay: suitable

storage temp.

−20°C

General description

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples.

Application

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi[1]. It has also been used to measure the ATP content in oocytes[1] and harderian gland(HG) tissue homogenates.

Preparation Note

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO4, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts.
Reconstitute with 50 mL of sterile water.

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This Item
FLAAMFLAASA2383
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer lyophilized powder

Sigma-Aldrich

FLAAB

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer

Adenosine 5′-triphosphate (ATP) assay mix lyophilized powder

Sigma-Aldrich

FLAAM

Adenosine 5′-triphosphate (ATP) assay mix

Adenosine 5′-triphosphate (ATP) disodium salt hydrate vial of ~1 mg ATP

Sigma-Aldrich

FLAAS

Adenosine 5′-triphosphate (ATP) disodium salt hydrate

Adenosine 5′-triphosphate disodium salt hydrate Grade I, ≥99%, from microbial

Sigma-Aldrich

A2383

Adenosine 5′-triphosphate disodium salt hydrate

technique(s)

activity assay: suitable

technique(s)

PCR: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000393676
0000393676
0000484811
0000484810
0000484810

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Global Trade Item Number

SKUGTIN
FLAAB-1VL04061838172716
FLAAB-10VL04061833296325
FLAAB-5VL04061838163530

Questions

  1. It seems that you are currently trying to perform the ATP assay (Sigma Cat# FLAA) on cardiac tissue. The kit appears to be working, as your standard curve is functioning, but you are obtaining very low numbers for your samples, significantly below your standard curve. Given that the heart should contain an abundance of ATP, you are concerned that your extraction protocol might be the issue. You are wondering if there is an extraction protocol available for this tissue for the mentioned kit.

    1 answer

    1. The low value observed in the sample may be due to several factors. Since the standard curve is working well, it is likely that the lysis buffer and/or homogenization condition used is not optimized for assay reading. To verify the possible presence of inhibiting substances, one could add a known amount of ATP from a standard solution to the homogenate to see if the reading increases accordingly. It's possible that the lysis buffer used was not optimal to yield an adequate amount of ATP for detection. Currently, there is no recommended cardiac tissue preparation protocol for use with this kit. As the kit measures only soluble ATP, it is highly recommended to clarify the homogenized extract by centrifugation to obtain a supernatant solution for use with this assay.

      Helpful?

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