Skip to Content
Merck

Skip To

D6665

2′,7′-Dichlorofluorescein

~90% purity (TLC) (85- 100% purity), powder

Synonym(s):

Dichlorofluorescein; Fluorescein 27

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice

About This Item

Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
Molecular Weight:
401.20
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
200-968-6
MDL number:
Beilstein/REAXYS Number:
58009
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

2′,7′-Dichlorofluorescein, suitable for use as an indicator in chloride titration, ~90% (TLC), powder

Quality Segment

assay

~90% (TLC)

form

powder

technique(s)

titration: suitable

mp

280 °C (dec.) (lit.)

solubility

ethanol: 25 mg/mL

density

0.79 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

General description

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).citation

Application

2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.[1]

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
41021735848D6883
technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

form

powder

form

powder

form

solid

form

powder

solubility

ethanol: 25 mg/mL

solubility

alcohol: passes test

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

−20°C


Still not finding the right product?

Explore all of our products under 2′,7′-Dichlorofluorescein


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

1–4 of 4 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. In what types of applications can 2´,7´-Dichlorofluorescein be used?

    1 answer
    1. Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).

      Helpful?

  3. What can be used to make a solution of 2´,7´-dichlorofluorescein?

    1 answer
    1. 2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.

      Helpful?

  4. What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?

    1 answer
    1. 2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.

      Helpful?

Reviews

No rating value

Active Filters