CS0013

Red Protease Detection Kit

Fluorescent protease assay, high sensitivity

• Synonym(s): Fluorescent Protease Assay Kit, Protease Assay Kit
• NACRES: NA.51
• UNSPSC Code: 12350000

Properties

• Quality Level: 200
• packaging: pkg of 1 kit
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.

Fluorescence excitation wavelength = 535 nm

Fluorescence emission wavelength = 595 nm

Application

Detection and quantitation of protease activity in samples. Useful for impurity testing.

Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.

Features and Benefits

• Fluorescence is pH-independent
• No separation or precipation steps are required
• High sensitivity and signal-to-noise
  

Other Notes

CS0013A Trypsin (1 mg/mL): 100 μL

CS0013B Protease AssayBuffer × 2: 30 mL

CS0013C TAMRA-Casein substrate: 280 μL

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable