C2978
Synonym(s):
Whole-cell protein extraction solution
| Size/SKU | Availability | Price |
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1 of 1
This Item | |||
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| form solution | form powder | form - | form - |
| storage temp. room temp | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
12 - Non Combustible Liquids
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The formulation is proprietary and below are the information that we can share.
This product does not contain any denaturants except for the "proprietary mild detergent" and the pH of the reagent is 7.6.
The reagent contains 10 mM salt and the detergent is known to be compatible with two dimensional gel electrophoresis.
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The Product Information Sheet recommends a protease inhibitor suitable for mammalian cell and tissue extracts, such as product numbers P8340, PIC0002, or I3786, as listed under "Reagents and Equipment Required but Not Provided." After adding the CelLytic M reagent, a 15-minute incubation on a shaker is recommended. The lysate should not be vortexed.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/033/c2978bul-ms.pdf
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The formulation does not contain EDTA or chelators. There is no known reason for it to be incompatible with Phos-Tag gel, but it has not been tested for this specific application.
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As mentioned in the 'DESCRIPTION' section, the composition of this product is considered proprietary and may not be shared. This product does not contain any denaturants except for a proprietary mild detergent. Lysates obtained with this product may be immediately run on a SDS PAGE after the addition of loading buffer.
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Is the product compatible with the BCA assay? Additionally, are there any denaturants in the formulation?
CelLytic™ M is suitable for Bradford Assay and BCA assays. For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent. The exact formulation is proprietary. This product may be diluted or dialyzed if it is found to have interference in your particular assay.
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What is the critical micelle concentration (CMC) of the product?
Unfortunately, the CMC value has not been tested for this product.
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Does the CelLytic M (Cat. No.: 2978) contain SDS, and if it does, at what concentration?
The product with the code C2978, CelLytic M, does not contain SDS. Instead, it contains a mild detergent in a buffer.
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Is the C2978 lysis buffer suitable for viral DNA extraction from cultured mammalian cells (fewer than 25,000 cells/ul) and subsequent qPCR?
The cellular DNA is centrifuged down and will not be present in the supernatant along with the proteins. To isolate the DNA, a different kit is necessary.
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We have not tested CelLytic for neutralizing HIV nor used in a BSL3 facility.
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Can Product C2978 CelLytic™ M be used for adherent cells?
Yes, CelLytic™ M can be used for both adherent cells and cells in suspension. Use 125 μl CelLytic™ M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μl for a 100 mm plate; 200-400 μl for a 35 mm plate.
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