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C0549

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

Synonym(s):

chloroform-isoamyl alcohol

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About This Item

NACRES:
NA.52
UNSPSC Code:
12190000
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form

liquid

Quality Segment

contains

0.6-1.0% ethanol as stabilizer

suitability

suitable for nucleic acid purification

application(s)

agriculture

General description

Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.

Application

Chloroform:Isoamyl alcohol 24:1 has been used:

  • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
  • for a single DNA precipitation step for high-quality DNA extraction.
  • for protein extraction and RNAse treatment.
  • in phenolic oxidation to separate phenolics from the aqueous portion.
  • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
  • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.

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This Item
M32658459844320021
Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

application(s)

agriculture

application(s)

-

application(s)

agriculture

application(s)

-

form

liquid

form

liquid

form

liquid

form

liquid

contains

0.6-1.0% ethanol as stabilizer

contains

-

contains

-

contains

-

suitability

suitable for nucleic acid purification

suitability

-

suitability

-

suitability

-


signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

185.0 °F

flash_point_c

85 °C



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A modified SDS-based DNA extraction method from raw soybean
Xia YC, et al.
Bioscience Reports, 39(2), BSR20182271-BSR20182271 (2019)
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
Healey A, et al.
Plant methods, 10(1), 1-8 (2014)
Defective insulin-stimulated GLUT4 translocation in brown adipocytes induces systemic glucose homeostasis dysregulation independent of thermogenesis in female mice
Picatoste B, et al.
Molecular Metabolism, 53 (2021)



Global Trade Item Number

SKUGTIN
C0549-1PT04061833450772
C0549-1QT04061837896583

Questions

1–2 of 2 Questions  
  1. Hi, what are the storage instructions for this product?

    1 answer
    1. This product may be stored at ambient temperature, tightly sealed, in a well-ventilated place.

      Helpful?

  2. Hello, what is the volume of this mixture please? And what is its shelf-life? Thanks. Katy

    1 answer
    1. This product is available in two package sizes, 1 pint (473 mL) and 1 quart (946 mL).

      This product is not assigned an expiration date or recommended retest date. Customers should routinely inspect products with no expiration date or recommended retest date to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

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