B6185

Bionic^™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

• Synonym(s): Sodium Borate Electrophoresis Buffer
• NACRES: NA.25
• UNSPSC Code: 41105319

Properties

• grade: Molecular Biology
• Quality Level: 200
• description: Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.
• form: solution
• concentration: 10 ×
• impurities: DNase, RNase and nickase, none detected
• pH: 8.0-8.5 (20-25 °C)
• storage temp.: room temp

Description

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:

• Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
• Use with TBA and TAE gels
• Preparation of agarose gels

Features and Benefits

• Faster run times
• Sharper bands
• Easy to dilute and use
• Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H319,H360FD
• pcodes: P202 - P264 - P280 - P305 + P351 + P338 - P308 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter