As indicated above under ‘Unit Definition’ the enzyme’s activity is tested at pH 6.0 and 25 °C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).
Optimal pH, pH and temperature stability of the enzyme, as well as effects of chemicals on its activity are addressed in the article below:
Production of Amylase in Liquid Culture by a Strain of Aspergillus oryzae
Kundu, A.K. and Das S.
Applied Microbiology, 19(4), 598-603 (1970)
Optimal pH and temperature ranges can also be found in the Brenda database:
https://www.brenda-enzymes.org/enzyme.php?ecno=3.2.1.1




