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Protein A–Agarose

Name: Protein A–Agarose
Brand: SIGMA

saline suspension

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:

41106500

NACRES:

NA.56

MDL number:

MFCD00165563

Form:

saline suspension

Biological source:

Staphylococcus aureus

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Properties

biological source

Staphylococcus aureus

Quality Level

200

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

Description

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.[1]

Application

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Protein A−Agarose has been used in the immunoprecipitation

of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
of prolyl hydroxylase domain 2 N27 cells
of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG.[1] Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

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This Item	P1406	P9269	P7155
Millipore P2545 Protein A–Agarose	Millipore P1406 Protein A–Agarose	Millipore P9269 Protein A–Agarose	Sigma-Aldrich P7155 Protein A from Staphylococcus aureus
biological source Staphylococcus aureus	biological source -	biological source -	biological source -
form saline suspension	form lyophilized powder	form lyophilized powder	form cell suspension
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
matrix activation cyanogen bromide	matrix activation cyanogen bromide	matrix activation cyanogen bromide	matrix activation -
matrix attachment amino	matrix attachment amino	matrix attachment amino	matrix attachment -