Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt has been used as an electron receptor, for use in demonstrating NADPH diaphorase and to stain glutathione reductase electrophoresis gels.
D1878
2,6-Dichloroindophenol sodium salt hydrate
Powder, BioReagent
Synonym(s):
2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, DCIP, DPIP
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About This Item
Empirical Formula (Hill Notation):
C12H6Cl2NNaO2 · xH2O
CAS Number:
Molecular Weight:
290.08 (anhydrous basis)
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
210-640-4
MDL number:
Beilstein/REAXYS Number:
3641229
Pricing and availability is not currently available.
Product Name
2,6-Dichloroindophenol sodium salt hydrate, suitable for vitamin C determination, BioReagent
product line
BioReagent
Quality Segment
form
powder
solubility
H2O: soluble 10 mg/mL, clear, blue to very deep blue
suitability
suitable for vitamin C determination
application(s)
diagnostic assay manufacturing
food and beverages
hematology
histology
storage temp.
room temp
SMILES string
O.[Na+].[O-]c1ccc(cc1)\N=C2/C=C(Cl)C(=O)C(Cl)=C2
InChI
1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1
InChI key
XHSOLXWCGCVQHE-UHFFFAOYSA-M
General description
2,6-Dichloroindophenol sodium salt hydrate is a blue redox dye.
Application
2,6-Dichloroindophenol sodium salt hydrate is suitable for vitamin C determination. 2,6-Dichloroindophenol (DCIP) may be used in the following studies:
- As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).
- To compose UVB specific dosimeter.[1]
- Quantification of vitamin C (L-ascorbic acid) in fruit juices.
- As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.[2]
- Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.[3]
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for vitamin C determination | suitability - | suitability - | suitability - |
| solubility H2O: soluble 10 mg/mL, clear, blue to very deep blue | solubility - | solubility H2O: 50 mg/mL | solubility - |
| application(s) diagnostic assay manufacturing | application(s) food and beverages | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form solid | form powder | form powder |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Other than use as a reagent for determination of Vitamin C, are there other reported uses of Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
1 answer-
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Are the absorbance maxima and the extinction coefficients available for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
1 answer-
Our Analytical Department has run absorbance spectra on many lots of D1878. Using 0.1 M phosphate buffer, pH 8.3 as the solvent, absorbance maxima in the range of 600-605 nm and a mM extinction coefficient of 18.0-19.7 have been reported.
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Has Sigma Aldrich performed solution stability studies for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
1 answer-
No. Unfortunately, we have not performed solution stability studies on this product.
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Are there published references for Dichloroindophenol sodium salt being used for the determination of Vitamin C?
1 answer-
Yes. Please see: W. Horowitz ed. Official Methods of Analysis of the AOAC Washington D.C. , 777, (1970) and K. Hiromi et al., Anal. Biochem. 101, 421, (1980).
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Has Product D1878, 2,6-Dichloroindophenol sodium salt hydrate, been used as a pH or redox indicator?
1 answer-
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt in acid is red and blue in alkali. The product is colorless when reduced.
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Are there substances known to interfere in Vitamin C determination?
1 answer-
Yes. Interfering substances are ferrous, stannous, and cuprous sulfite or thiosulfate ions, and SO2.
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What is the solubility for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
1 answer-
During quality control testing, Sigma Aldrich tests solubility at 10 mg/mL in water. Conn's Biological Stains, 10th Edition states that dichloroindophenol sodium salt is soluble up to 3% in water and up to 2% in ethanol.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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