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CS1000

Superoxide Anion Assay Kit

sufficient for 100 assays (96 well plates)

Synonym(s):

Superoxide Detection Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 assays (96 well plates)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Application

The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2− anion status in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma.

Biochem/physiol Actions

The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.

Analysis Note

The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal.

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Superoxide Anion Assay Kit sufficient for 100 assays (96 well plates)

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Histone Deacetylase Assay Kit, Fluorometric sufficient for 100 assays (96 well plates)

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Histone Deacetylase Assay Kit, Fluorometric

Glutathione Assay Kit sufficient for 700 assays

Sigma-Aldrich

CS0260

Glutathione Assay Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 100 assays (96 well plates)

usage

sufficient for 100 assays

usage

sufficient for 100 assays (96 well plates)

usage

sufficient for 700 assays

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

Kit Components Only

Product No.
Description
  • Assay Buffer 25 mL
  • Assay Medium 20 mL
  • Enhancer Solution, 40 mM 500 μL
  • Luminol Solution 500 μL
  • Xanthine Solution 500 μL
  • Xanthine Oxidase .5 U
  • Superoxide Dismutase from bovine liver, 4 units/μL 400 U

Storage Class

10 - Combustible liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000160563
0000160563
0000114035
089M4044V
11190616

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Global Trade Item Number

SKUGTIN
CS1000-1KT04061833553176

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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