CPISO

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

• Synonym(s): Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• Quality Segment: 200
• technique(s): centrifugation: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

Application

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.

The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.

Features and Benefits

• Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
• Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
• Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
• Convenient format - Saves time and reduces waste

Kit Components Also Available Separately

• Chloroplast Isolation Buffer 5x (CIB) 500 mL

  SDS
• A8022

  Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 g

  SDS
• Chloroplast Isolation Buffer 5x (CIB) 4 ea

  SDS
• P4937

  Percoll^®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mL

  SDS

Safety Information

• Storage Class: 10 - Combustible liquids