A8919

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti Goat Hrp Sigma
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162340
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: DB, ELISA (d), IHC (p), WB CL
• Citations: 66

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:30,000, dot blot: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot (chemiluminescent): 1:160,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit anti-Goat IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative.

Immunogen

Purified goat IgG

Application

Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313