Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
Biological source:
human plasma
Form:
lyophilized (clear, colorless solution after reconstitution)
Technique(s):
cell culture | mammalian: suitable
Mol wt:
440 kDa
Assay:
>95% (SDS-PAGE)
Pricing and availability is not currently available.
biological source
human plasma
sterility
non-sterile; 0.2 μm filtered
assay
>95% (SDS-PAGE)
form
lyophilized (clear, colorless solution after reconstitution)
mol wt
440 kDa
packaging
pkg of 1 mg (11051407001), pkg of 5 mg (11080938001)
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
General description
Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.[1]
Application
Biochem/physiol Actions
Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).
Physical form
Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin
Preparation Note
Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
1 of 1
This Item | |||
|---|---|---|---|
| biological source human plasma | biological source human plasma | biological source human | biological source mouse |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, inhibition assay: suitable, western blot: suitable |
| sterility non-sterile; 0.2 μm filtered | sterility non-sterile; 0.2 μm filtered | sterility - | sterility - |
| assay >95% (SDS-PAGE) | assay 95% (SDS-PAGE) | assay - | assay - |
| form lyophilized (clear, colorless solution after reconstitution) | form lyophilized (clear, colorless solution after reconstitution) | form liquid | form - |
| mol wt 440 kDa | mol wt 440 kDa | mol wt - | mol wt - |
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Roberta Bulla et al.
Nature communications, 7, 10346-10346 (2016-02-03)
Complement C1q is the activator of the classical pathway. However, it is now recognized that C1q can exert functions unrelated to complement activation. Here we show that C1q, but not C4, is expressed in the stroma and vascular endothelium of
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Benjamin N Rollo et al.
F1000Research, 4, 113-113 (2015-06-13)
The avian enteric nervous system (ENS) consists of a vast number of unusually small ganglia compared to other peripheral ganglia. Each ENS ganglion at mid-gestation has a core of neurons and a shell of mesenchymal precursor/glia-like enteric neural crest (ENC)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11080938001 | 04061838698292 |
| 11051407001 | 04061838697493 |