BSAV-RO

Bovine Serum Albumin

≥98.5%, New Zealand origin

• Synonym(s): Bovine Serum Albumin Fraction V
• UNSPSC Code: 12352202
• NACRES: NA.54
• Biological source: bovine
• Assay: ≥98.5%
• Form: lyophilized
• Technique(s): ELISA: suitable, Southern blotting: suitable, western blot: suitable
• Impurities: <0.0003 Lithium (Flame photometry), <0.001 Iron (bathophenanthraline), <0.002 P (inorganic), <0.003 Heavy metals (as Pb), <0.003 Magnesium (xylidyl blue), <0.005 Glycerol (enzyme), <0.006 potassium (Flame photometry), <0.02 Calcium ( o-cresolphthalein), <0.05 glucose (enzyme), <0.1 L-lactate, <0.15 chloride (mercurom), <0.5 Sodium (flame photometry), <100 microorganisms (organism/g), <5 water

Properties

• biological source: bovine
• assay: ≥98.5%
• form: lyophilized
• mol wt: M_r =68 kDa
• packaging: pkg of 1 kg (10735108001), pkg of 100 g (10735086001), pkg of 50 g (10735078001), pkg of 500 g (10735094001)
• manufacturer/tradename: Roche
• origin: New Zealand origin
• technique(s): ELISA: suitable, Southern blotting: suitable, western blot: suitable
• impurities: <0.0003 Lithium (Flame photometry), <0.001 Iron (bathophenanthraline), <0.002 P (inorganic), <0.003 Heavy metals (as Pb), <0.003 Magnesium (xylidyl blue), <0.005 Glycerol (enzyme), <0.006 potassium (Flame photometry), <0.02 Calcium ( o-cresolphthalein), <0.05 glucose (enzyme), <0.1 L-lactate, <0.15 chloride (mercurom), <0.5 Sodium (flame photometry), <100 microorganisms (organism/g), <5 water
• absorption: ≤0.4 at 550 nm

Description

General description

For standard applications.

Application

Bovine Serum Albumin Fraction V is suitable for standard applications such as:

• Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.[1]
• Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
• Reducing agent
• Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots[2]
• Media supplement for media that require addition of protein
• Carrier protein
• Standard protein in gel electrophoresis and protein determination[3]

Preparation Note

Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

Analysis Note

Purity: ≤95% protein, ≤5% H₂O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable